sunrise elisa plate reader (Tecan Systems)
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Sunrise Elisa Plate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 10445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 10445 article reviews
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Enzyme-linked Immunosorbent Assay:Article Title: The paradoxical protective effect of chronic stress on advanced Alzheimer's disease pathology. Article Snippet: Background: Core pathology in Alzheimer’s disease (AD) includes amyloid-β (Aβ) deposition, gliosis, and eventual neuronal loss.. Depression during midlife increases the risk of developing AD at late life.. Late-life depression is highly prevalent among AD patients, but its role in AD pathogenesis is unclear, and specifically whether it pushes the brain with established AD pathology towards degeneration. Article Title: Blood-free detection strategy based on swellable microneedle patches for neonatal infection and adult health management Article Snippet: .. The stopping solution was then added, and an absorbance at 445 nm was immediately recorded using an Article Title: Antioxidant and Anticancer Activities of Water Extracts from Flowers, Leaves and Stems of In Vitro Cultivated and Wild-Growing Marrubium vulgare Plants Article Snippet: .. The absorbance of the samples was measured at 570 nm using an Article Title: Human assembloids recapitulate periportal liver tissue in vitro. Article Snippet: For sandwich cultures, fresh isolated PHHs were plated onto collagen as above and overlayed with a second collagen layer (1.2 mg ml–1; RatCol collagen, Advanced Biomatrix) and cultured for 7 days in William’s E medium supplemented with CM4000 cell maintenance supplement (ThermoFisher Scientific). .. To determine albumin secretion, supernatant from 24 h was collected and the amount of albumin was determined using a human-specific albumin ELISA kit (Assay Pro) following the manufacturer’s instructions on an Article Title: Human assembloids recapitulate periportal liver tissue in vitro Article Snippet: For sandwich cultures, fresh isolated PHHs were plated onto collagen as above and overlayed with a second collagen layer (1.2 mg ml –1 ; RatCol collagen, Advanced Biomatrix) and cultured for 7 days in William’s E medium supplemented with CM4000 cell maintenance supplement (ThermoFisher Scientific). .. To determine albumin secretion, supernatant from 24 h was collected and the amount of albumin was determined using a human-specific albumin ELISA kit (Assay Pro) following the manufacturer’s instructions on an Article Title: Antioxidant and Anticancer Activities of Water Extracts from Flowers, Leaves and Stems of In Vitro Cultivated and Wild-Growing Marrubium vulgare Plants Article Snippet: .. The absorbance of the samples was measured at 570 nm using an Article Title: Porcine placental extract supplementation enhances testosterone levels and semen quality without adverse effects in Beagle dogs. Article Snippet: Porcine placental extract contains bioactive compounds with several biological properties.. However, its effects on canine reproductive performance and systemic safety remain unclear.. This study aimed to investigate the effects of porcine placental extract supplementation on canine semen quality, testosterone levels, systemic toxicity, antioxidant capacity, and diet palatability. Article Title: Computational modeling of platelet activation signatures in response to diverse immune and hemostatic agonists. Article Snippet: The soluble α-granule protein content of platelet supernatants was quantified using Luminex technology with the following panel of human cytokine/chemokine magnetic beads: HCYTOMAG-60K, HCYP2MAG62K, and HCYP3MAG-63K (Millipore, Molsheim, France) as described previously.16 A canonical soluble δ granule molecule, content of platelet supernatants, serotonin (outside of the panel described above), was quantified by ELISA (IBL International, Hamburg, Germany). .. Absorbance at 450 nm (or 405 nm for serotonin) was measured using an Software:Article Title: Computational modeling of platelet activation signatures in response to diverse immune and hemostatic agonists. Article Snippet: The soluble α-granule protein content of platelet supernatants was quantified using Luminex technology with the following panel of human cytokine/chemokine magnetic beads: HCYTOMAG-60K, HCYP2MAG62K, and HCYP3MAG-63K (Millipore, Molsheim, France) as described previously.16 A canonical soluble δ granule molecule, content of platelet supernatants, serotonin (outside of the panel described above), was quantified by ELISA (IBL International, Hamburg, Germany). .. Absorbance at 450 nm (or 405 nm for serotonin) was measured using an |
![CD8 + T cells from individuals with hoFH validate a role of LDLR signaling for CD8 + T cell function (A) A schematic overview of the LDLR protein domains and LDLR-adaptor protein 1 (LDLRAP1), highlighting the hoFH mutations included in the experiments with blue reversed triangles. The empty reverse triangle shapes represent hoFH patients with mutations in the LDLR, while the filled reverse triangle shape represents hoFH patients with mutations in the LDLRAP1. (B) Cell surface LDLR gMFI levels on CD8 + T cells from hoFH patients ( n = 5) and healthy controls (HCs) ( n = 5). Cells were activated for the indicated duration of time with anti-CD3/CD28 Dynabeads and a cytokine mix (interleukin [IL]-2, IL-7, and IL-15). (C) Graphical illustration of the LDL-pHrodo uptake experiment. (D) Uptake of LDL-pHrodo measured by flow cytometry, where CD8 + T cells were activated for 24 h, followed by a 2-h culturing in lipoprotein-deprived medium and 2-h incubation with the LDL-pHrodo complex (20 μg/mL). Where indicated, anti-LDLR (5 μg/mL) was added when cells were cultured in lipoprotein-deprived medium. One-way ANOVA with Šídák’s multiple comparisons test, where ∗∗∗∗ p < 0.0001 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (E–H) Cells were activated for 72 h with anti-CD3/CD28 Dynabeads and cytokine mix (IL-2, IL-7, and IL-15). (E) Intracellular Ki67 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (F) Cell surface ICAM-1 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (G) Intracellular granzyme B levels measured with flow cytometry. Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). (H) Secreted granzyme B levels measured with <t>ELISA.</t> Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6826/pmc12936826/pmc12936826__gr5.jpg)